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Search results: akt

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Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts from K562, using Akt (Phospho-Ser473) Antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetAKT1
  • SizePrice
Western blot analysis of extracts from HeLa cells, using Akt (Ab-473) Antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetAKT1
  • SizePrice
Western Blot of Rabbit anti-AKT pS473 antibody. Lane 1: nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase. Load: 35 ug per lane. Primary antibody: AKT pS473 antibody at 1:200 dilution for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~56 kDa for AKT pS473. Other band (s): unspecific.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Rabbit anti-Akt phospho T308 antibody. Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Other band (s): none.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
WB of detection of unphosphorylated and phosphorylated Rabbit Anti-AKT antibody. Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Rabbit anti-Akt (pan) and mouse anti-Akt pS473 specific antibodies at 1:1000. Secondary antibody: DyLight™ 549 conjugated anti-rabbit IgG (green) and DyLight™ 649 conjugated anti-mouse IgG (red)at 1:10,000.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Rabbit Anti-AKT Antibody. Lane 1: HeLa cells. Lane 2: H2O2 treated MCF7 cells. Lane 3: Recombinant AKT. Lane 4: A431 cells. Load: 35 ug per lane. Primary antibody: AKT antibody at 1:300 for overnight at 4°C. Secondary antibody: IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature. Block: 1% BLOTTO overnight at 4°C. Predicted/Observed size: ~55-60 kDa for AKT. Other band (s): unspecific.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetAKT1
  • SizePrice
WB of Mouse Anti-Akt pS473 antibody. Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Load: 10 ug per lane. Primary antibody: rabbit anti-Akt (pan) and mouse anti-Akt pS473 antibodies at 1:400. Secondary antibody: DyLight™ 549 conjugated anti-rabbit IgG (green) and DyLight™ 649 conjugated anti-mouse IgG (red) at 1:10,000.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice
WB of Mouse anti-AKT pT308 antibody. Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT pT308 antibody at a 1:4,000 dilution. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) (p/n 610-1303) was used at a 1:40,000 dilution for 1 h at 4°C. Block: 3% BSA (p/n BSA-30) in TBS for 30 min at RT.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT1
  • SizePrice
WB of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4°C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band (s): none.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Primate, Rat
TargetAKT1
  • SizePrice

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