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Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (GTX132615) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
AKT (phospho Ser473) antibodyGTX132615
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (GTX133175) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
AKT (phospho Thr450) antibodyGTX133175
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Rabbit anti-Akt phospho T308 antibody (GTX28933). Lane 1: GST tagged AKT1 un-active recombinant protein. Lane 2: GST tagged AKT1 active recombinant protein. Load: 50 ng per lane. Primary antibody: Akt phospho T308 antibody at 1:1,000 for overnight at 4oC. Secondary antibody: DyLight? 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other band(s): none.
Product group Antibodies
References
GeneTex
AKT (phospho Thr308) antibodyGTX28933
ApplicationsDot Blot, Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
GeneTex
AKT pathway Antibody PanelGTX300089
  • SizePrice
Product group Chemicals
Biosynth
Akt/SKG Substrate PeptideCRB1000597
Molecular Weight817.5 g/mol
  • SizePrice
Chemical Structure
Product group Chemicals
SYNkinase
Akt-I-1 [473382-39-7] [473382-39-7]SYN-1005
CAS Number473382-39-7
Estimated Purity>95%
Molecular Weight378.5
  • SizePrice
Product group Assays
SignalChem
Modified AKT Substrate IIA05-58C
  • SizePrice
Product group Assays
Assay Genie
Rat Protein Kinase B/PKB/AKT ELISA KitRTFI01064
Assay Sample TypeSerum, Plasma
ReactivityRat
  • SizePrice
Product group Antibodies
Bioss
AKTBSM-63324R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Chemicals
Biosynth
Akt-I-1,2 HCl [473382-50-2]YTA38250
CAS Number473382-50-2
Molecular Weight339.43 g/mol
  • SizePrice
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody [HL2871] (GTX640148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
AKT (phospho Ser473) antibody [HL2871]GTX640148
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
Specificity test analysis was performed by coating the plate with recombinant AKT1 peptide and AKT1 (phospho Ser473) peptide (1000-15.63 ng/mL). Coated protein was probed with AKT (phospho Ser473) antibody [HL2971] (GTX640358) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
Product group Antibodies
GeneTex
AKT (phospho Ser473) antibody [HL2971]GTX640358
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice
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