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Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse Raw264.7 whole cell lysates, Lane 3: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 150 kDa. The expected band size for CEACAM1 is at 57,50,37,30 kDa.
Product group Antibodies
Boster Bio
Anti-Ceacam1 Antibody Picoband(r)A00923-3-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetCeacam1
  • SizePrice
Figure 1. Western blot analysis of CEACAM1 using anti-CEACAM1 antibody (A00923-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse Raw264.7 whole cell lysates, Lane 3: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM1 antigen affinity purified polyclonal antibody (Catalog # A00923-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEACAM1 at approximately 150 kDa. The expected band size for CEACAM1 is at 57,50,37,30 kDa.
Product group Antibodies
Boster Bio
Anti-Ceacam1 Antibody Picoband(r)A00923-3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetCeacam1
  • SizePrice
Anti-CEACAM5 Antibody [IHC543]
Product group Antibodies
Antibodies.com
Anti-CEACAM5 Antibody [IHC543]A324392
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-CEACAM5 Antibody [CB30]A279990
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • SizePrice
Product group Antibodies
Biorbyt
Anti-CEACAM4 AntibodyORB666256
ApplicationsWestern Blot
TargetCEACAM4
  • SizePrice
Figure 1. Western blot analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for CEACAM5 at approximately 130-200KD. The expected band size for CEACAM5 is at 77KD.
Product group Antibodies
Boster Bio
Anti-CEACAM5 Antibody Picoband(r)A00356-DYLIGHT550
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCEACAM5
  • SizePrice
Figure 1. Western blot analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for CEACAM5 at approximately 130-200KD. The expected band size for CEACAM5 is at 77KD.
Product group Antibodies
Boster Bio
Anti-CEACAM5 Antibody Picoband(r)A00356-DYLIGHT594
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCEACAM5
  • SizePrice
Figure 1. Western blot analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for CEACAM5 at approximately 130-200KD. The expected band size for CEACAM5 is at 77KD.
Product group Antibodies
Boster Bio
Anti-CEACAM5 Antibody Picoband(r)A00356-FITC
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCEACAM5
  • SizePrice
Figure 1. Western blot analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for CEACAM5 at approximately 130-200KD. The expected band size for CEACAM5 is at 77KD.
Product group Antibodies
Boster Bio
Anti-CEACAM5 Antibody Picoband(r)A00356-HRP
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCEACAM5
  • SizePrice
Figure 1. Western blot analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for CEACAM5 at approximately 130-200KD. The expected band size for CEACAM5 is at 77KD.
Product group Antibodies
Boster Bio
Anti-CEACAM5 Antibody Picoband(r)A00356-IFLUOR647
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCEACAM5
  • SizePrice
Figure 1. Western blot analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for CEACAM5 at approximately 130-200KD. The expected band size for CEACAM5 is at 77KD.
Product group Antibodies
Boster Bio
Anti-CEACAM5 Antibody Picoband(r)A00356-PE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCEACAM5
  • SizePrice
Figure 1. Western blot analysis of CEACAM5 using anti-CEACAM5 antibody (A00356). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for CEACAM5 at approximately 130-200KD. The expected band size for CEACAM5 is at 77KD.
Product group Antibodies
References
Boster Bio
Anti-CEACAM5 Antibody Picoband(r)A00356
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetCEACAM5
  • SizePrice
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