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Search results: ifng

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Product group Antibodies
Antibodies.com
ApplicationsELISA
ReactivityHuman
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Anti-Interferon gamma Antibody [IFNG/3996R]
Product group Antibodies
Antibodies.com
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
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Product group Proteins / Signaling Molecules
Antibodies.com
ApplicationsOther Application
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Product group Antibodies
MyBioSource
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Product group Antibodies
MyBioSource
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Product group Antibodies
MyBioSource
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Figure 1. Sandwich ELISA - Recombinant rat Interferon gamma protein standard curve. Use in combination with reagents from Rat Interferon gamma ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0374).
Product group Antibodies
Boster Bio
ApplicationsELISA
ReactivityRat
TargetIfng
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Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIfng
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Product group Assays
Biorbyt
ReactivitySheep
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Product group Assays
Biorbyt
ReactivityMonkey
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Product group Assays
Biorbyt
ReactivityBovine
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Product group Assays
Biorbyt
ReactivityMonkey
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