More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/

Search results: lacZ

13 to 24 of 52 products
Sort by
Product group Antibodies
Assay Genie
lacZ Antibody (PACO56804)PACO56804
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Assay Genie
lacZ Monoclonal Antibody (MACO0616)MACO0616
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • SizePrice
Product group Antibodies
Cusabio
lacZ Antibody, HRP conjugatedCSB-PA009476LB01ENV
ApplicationsELISA
ReactivityBacteria
  • SizePrice
Product group Antibodies
Cusabio
lacZ Antibody, FITC conjugatedCSB-PA009476LC01ENV
ReactivityBacteria
  • SizePrice
Product group Antibodies
Cusabio
lacZ Antibody, Biotin conjugatedCSB-PA009476LD01ENV
ApplicationsELISA
ReactivityBacteria
  • SizePrice
Figure 1. Western blotting using Fluorescein conjugated anti-Beta-galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to Nitrocellulose and blocking using blocking buffer for fluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase (. F) diluted to 1/10,000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® Imaging System. Other detection systems will yield similar results.
Product group Antibodies
OriGene
lacZ Rabbit Polyclonal AntibodyR1064
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityBacteria
TargetlacZ
  • SizePrice
Western blot using Beta-galactosidase antibody. shows detection of a band at ~117 kDa (lane 1) corresponding to the protein present in partially purified preparations. Approximately 50 ng of protein was separated on a 4-20% Tris-Glycine gel by SDSPAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1/1,000. Reaction occurred overnight at 4°C followed by washes and reaction with a 1/10,000 dilution of IRDye800 (TM) conjugated goat-a-rabbit IgG [H&L] for 45 min at RT (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). RDye800 (TM) fluorescence image was captured using the Odyssey (R) infrared imaging system developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product group Antibodies
OriGene
lacZ Rabbit Polyclonal AntibodyR1064PS
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityBacteria
TargetlacZ
  • SizePrice
lacZ Chicken Polyclonal Antibody
Product group Antibodies
OriGene
lacZ Chicken Polyclonal AntibodyAP31768PU-N
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetlacZ
  • SizePrice
Product group Antibodies
OriGene
lacZ Rabbit Polyclonal AntibodyAP21258BT-N
ApplicationsImmunoDiffusion, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, RadioImmunoAssay
ReactivityBacteria
TargetlacZ
  • SizePrice
Product group Antibodies
OriGene
lacZ Rabbit Polyclonal AntibodyR1064HRPS
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityBacteria
TargetlacZ
  • SizePrice
Western blot of rabbit anti-Beta-galactosidase (E. coli) antibody biotin conjugated. Lane 1: Beta-galactosidase. Load: 50 ng per lane. Primary antibody: Beta-galactosidase (E. coli) antibody biotin conjugated. R1064B) at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer ( for fluorescent Western blotting) for 30 min at RT. Predicted/observed size: 116 kDa, 116 kDa for Beta-galactosidase.
Product group Antibodies
OriGene
lacZ Rabbit Polyclonal AntibodyR1064BS
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityBacteria
TargetlacZ
  • SizePrice
Western blotting using fluorescein conjugated anti-Beta-galactosidase tag antibody shows a band at ~117 kDa (lanes 1-3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for bluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase. R1064F diluted to 1/10,000. Reaction occurred for 2 hours at RT. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® imaging system. Other detection systems will yield similar results.
Product group Antibodies
OriGene
lacZ Rabbit Polyclonal AntibodyR1064FS
ApplicationsImmunoFluorescence, Western Blot
ReactivityBacteria
TargetlacZ
  • SizePrice
12345

Didn't find what you were looking for?

Search through our product groups to find the right product

Back to overview
AntibodiesChemicalsProteins / Signaling MoleculesAssaysDNA / RNA / VectorsCell CultureGenome Editing and EngineeringMolecular BiologyLysates / ExtractsExpressionLabware / AccesoriesBody ProductsTechnique Specific ProductsArrays / Slides