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Search results: p53 TP53

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Western blot analysis of extracts of HeLa cells, using Phospho-p53-S46 antibody (TA382722) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetTP53
  • SizePrice
Western blot analysis of extracts of HT-29 cells, using Phospho-p53-S33 pAb (TA382723) at 1:2000 dilution or p53 antibody (A3185).HT-29 cells were treated by Nocodazole (100ng/mL) for 16 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-p53-S392 antibody (TA382724) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37°C for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37°C for 20 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Enhanced Kit . - Exposure time: 1s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
Western blot analysis of extracts of 293T cells, using Phospho-Tp53-S15 antibody (TA382725) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 1s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetTP53
  • SizePrice
Western blot analysis of extracts of MCF7 cells, using Phospho-Tp53-S6 antibody (TA382726) at 1:1000 dilution.MCF7 cells were treated by ATP(5 mM) at 30°C for 1 hour. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 90s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-p53-S37 antibody (TA382728) at 1:1000 dilution.Both 293T cells and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 1s.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
Western blot analysis of extracts of HeLa cells, using Phospho-p53-S367 antibody (TA382729) at 1:500 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice
Western blot analysis of extracts of C6 cells, using Phospho-p53-T81 antibody (TA382730) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetTP53
  • SizePrice
WB: The cell lysate derived from UV stimulated Hela cells was immunoprobed by Rabbit anti-p53 (pS15) antibody at 1:500. An immunoreactive band is observed around ~ (Lane 1). The lane 2 is a negative control.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
WB: The cell lysate derived from UV stimulated Hela cells was immunoprobed by Rabbit anti-p53 (pS37) at 1:500. An immunoreactive band is observed around ~53kDa
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
Western blot analysis of extracts from COS7 cells, using p53 (Ab-15) Antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Western blot analysis of extracts from 293 cells, using p53 (Ab-46) Antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice