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Search results: p53 TP53

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Western blot analysis of extracts of MCF7 cells, using Phospho-Tp53-S6 antibody (TA382726) at 1:1000 dilution.MCF7 cells were treated by ATP(5 mM) at 30°C for 1 hour. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 90s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-p53-S37 antibody (TA382728) at 1:1000 dilution.Both 293T cells and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 1s.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
Western blot analysis of extracts of HeLa cells, using Phospho-p53-S367 antibody (TA382729) at 1:500 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice
Western blot analysis of extracts of C6 cells, using Phospho-p53-T81 antibody (TA382730) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetTP53
  • SizePrice
WB: The cell lysate derived from UV stimulated Hela cells was immunoprobed by Rabbit anti-p53 (pS15) antibody at 1:500. An immunoreactive band is observed around ~ (Lane 1). The lane 2 is a negative control.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
WB: The cell lysate derived from UV stimulated Hela cells was immunoprobed by Rabbit anti-p53 (pS37) at 1:500. An immunoreactive band is observed around ~53kDa
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
Western blot analysis of p53 phosphorylation expression in HU treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetTP53
  • SizePrice
Western blot analysis of p53 phosphorylation expression in UV treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
Western blot analysis of p53 phosphorylation expression in ovarycancer whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice
Western blot analysis of p53 phosphorylation expression in Adriamycin treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice
Western blot analysis of p53 phosphorylation expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice
Western blot analysis of p53 phosphorylation expression in UV treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice