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Search results: zebrafish

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-PCNA Mouse Monoclonal Antibody (SPM350).
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityChicken, Drosophila, Human, Monkey, Mouse, Porcine, Rat, Yeast, Zebra Fish
TargetPCNA
  • SizePrice
Formalin-fixed, paraffin-embedded human Erdheim-Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNF706 + P/T2).
Product group Antibodies
References
Boster Bio
ApplicationsImmunoHistoChemistry
ReactivityCanine, Feline, Human, Mouse, Rabbit, Rat, Zebra Fish
TargetTNF
  • SizePrice
Figure 1. Western blot analysis of beta Actin/ACTB using anti-beta Actin/ACTB antibody (PA1872). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta Actin/ACTB antigen affinity purified polyclonal antibody (Catalog # PA1872) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta Actin/ACTB at approximately 42 kDa. The expected band size for beta Actin/ACTB is at 42 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot
ReactivityChicken, Hamster, Human, Monkey, Mouse, Rat, Zebra Fish
TargetACTB
  • SizePrice
Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityChicken, Human, Monkey, Mouse, Rat, Zebra Fish
TargetGAPDH
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Product group Antibodies
Boster Bio
ReactivityZebra Fish
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Zebrafish kmo AntibodyDZ41201-DYLIGHT488
ReactivityZebra Fish
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Zebrafish kmo AntibodyDZ41201-DYLIGHT550
ReactivityZebra Fish
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Zebrafish kmo AntibodyDZ41201-DYLIGHT594
ReactivityZebra Fish
  • SizePrice
Product group Antibodies
Boster Bio
ReactivityZebra Fish
  • SizePrice
Product group Antibodies
Boster Bio
ReactivityZebra Fish
  • SizePrice
Product group Antibodies
Boster Bio
ReactivityZebra Fish
  • SizePrice
Product group Antibodies
Boster Bio
ReactivityZebra Fish
  • SizePrice