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WB analysis of various sample lysates using GTX66073 SEC13L1 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66073 SEC13L1 antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66073 SEC13L1 antibody. Dilution : 1:3000 Loading : 25microg per lane

SEC13L1 antibody

GTX66073
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSEC13
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Overview

  • Supplier
    GeneTex
  • Product Name
    SEC13L1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6396
  • Target name
    SEC13
  • Target description
    SEC13 homolog, nuclear pore and COPII coat complex component
  • Target synonyms
    D3S1231E; GATOR complex protein SEC13; npp-20; protein SEC13 homolog; SEC13 homolog, nuclear pore and COPII coating complex component; SEC13L1; SEC13-like protein 1; SEC13R; SEC13-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55735
  • Protein Name
    Protein SEC13 homolog
  • Scientific Description
    The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEC13L1/SEC13 using anti-SEC13L1/SEC13 antibody (A04346-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat small intestine tissue lysates, Lane 7: mosue brain tissue lysates, Lane 8: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC13L1/SEC13 antigen affinity purified polyclonal antibody (Catalog # A04346-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC13L1/SEC13 at approximately 36 kDa. The expected band size for SEC13L1/SEC13 is at 36 kDa.
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