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SEC23IP Polyclonal Antibody

CAC12968
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSEC23IP
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Overview

  • Supplier
    Biomatik
  • Product Name
    SEC23IP Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11196
  • Target name
    SEC23IP
  • Target description
    SEC23 interacting protein
  • Target synonyms
    intracellular phospholipase A1 beta; iPLA1beta; MSTP053; P125; P125A; SEC23-interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6Y8
  • Protein Name
    SEC23-interacting protein
  • Scientific Description
    The SEC23IP Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEC23IP using anti-SEC23IP antibody (A09955-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 292T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC23IP antigen affinity purified polyclonal antibody (Catalog # A09955-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC23IP at approximately 125 kDa. The expected band size for SEC23IP is at 111 kDa.
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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: SEC23IP antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 112, 103 kDa Observed band size: 112 kDa
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