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Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120). Lane M: 3 microl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product group Antibodies
OriGene
Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-AdsorbedTA398043
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120). Lane M: 3 microl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product group Antibodies
OriGene
Rat IgG (H&L) Antibody Alkaline Phosphatase ConjugatedTA398044
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120). Lane M: 3 microl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product group Antibodies
OriGene
Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-AdsorbedTA398045
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120). Lane M: 3 microl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product group Antibodies
OriGene
Rat IgG (H&L) Antibody Biotin ConjugatedTA398046
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120). Lane M: 3 microl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product group Antibodies
OriGene
Rat IgG (H&L) Antibody Biotin Conjugated Pre-AdsorbedTA398047
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120). Lane M: 3 microl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product group Antibodies
OriGene
Rat IgG (H&L) Antibody Texas Red(tm) ConjugatedTA398048
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120). Lane M: 3 microl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (DONKEY) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) (p/n 612-701-120) 1:10,000 for 60 min at RT. Secondary Antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product group Antibodies
OriGene
Rat IgG (H&L) Antibody Texas Red(tm) Conjugated Pre-AdsorbedTA398049
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA Results of Rabbit Anti-Sheep IgG Antibody Peroxidase Conjugate (Min X Human Serum Proteins) tested against purified Sheep IgG Min X Hu HRP. Each well was coated in duplicate with 1.0 microg of Sheep IgG (p/n 013-0102). The working dilution is 1:57000. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.
Product group Antibodies
OriGene
Anti-Sheep IgG HRP Conjugated Antibody Pre-AdsorbedTA398050
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA results of purified Rabbit anti-Sheep IgG biotin conjugated antibody Min X human serum tested against purified Sheep IgG. Each well was coated in duplicate with 1.0 microg of sheep IgG (red line). The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate 1:10, 000, and TMB-1000 substrate.
Product group Antibodies
OriGene
Sheep IgG (H&L) Secondary Antibody Biotin Conjugated Pre-AdsorbedTA398051
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
Sheep IgG (H&L) Antibody Rhodamine Conjugated Pre-AdsorbedTA398052
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
Sheep IgG (H&L) Antibody Rhodamine ConjugatedTA398053
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA Results of Donkey Anti-Sheep IgG Antibody (Min X Ch GP Ham Hs Hu Ms Rb Rt Serum Proteins) tested against purified Sheep IgG Mx8. Each well was coated in duplicate with 1.0 microg of Sheep IgG (p/n 013-0102) [Red Line], Chicken IgG (p/n 003-0102) [Green Line], Guinea Pig IgG (p/n 006-0102) [Blue Line], Hamster IgG (p/n 007-0102) [Purple Line], Horse IgG (p/n 008-0102), Human IgG (p/n 009-0102), Mouse IgG (p/n 010-0102), Rabbit IgG (p/n 011-0102), and Rat IgG (p/n 012-0102). The working dilution of Sheep IgG MX8 is 1:49,000. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Goat Anti-Donkey IgG HRP conjugated (p/n 616-4302), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
Sheep IgG (H&L) Antibody Pre-adsorbedTA398054
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
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