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Western Blot of Goat anti-Swine IgG antibody. Lane 1: Swine IgG (p/n 014-0102). Lane 2: none. Load: 100 ng per lane. Primary antibody: Swine Antibody at 1:1,000 o/n at 4°C . Secondary antibody: DyLight™ 649 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 28 kDa for Swine IgG. Other band(s): none.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Dot Blot of Fluorescien Conjugated Goat anti-Swine Antibody. Antigen: Swine IgG. Load: 200 ng followed by a 3-fold serial dilution. Primary antibody: none. Secondary antibody: Fluorescien swine secondary antibody at 1:1,000 for 60 min at RT.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Peroxidase Conjugated Goat Anti-Swine IgG Secondary Antibody. Lane 1: Swine IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Swine IgG. Other band(s): None.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Dot Blot of Goat anti-Swine IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Swine IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Swine IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Western Blot of Biotin conjugated Goat anti-Swine antibody. Lane 1: Swine IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: Biotin conjugated swine antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa for Swine IgG. Other band(s): none.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA Results of Goat Anti-Donkey IgG Antibody tested against purified Donkey IgG. Each well was coated in duplicate with 1.0 microg of Donkey IgG (p/n 016-0102). The working dilution is 1:38,200. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA Results of Goat Anti-Donkey IgG Antibody Peroxidase Conjugate tested against purified Donkey IgG HRP. Each well was coated in duplicate with 1.0 microg of Donkey IgG (p/n 016-0102). The working dilution is 1:188,000. The starting dilution of antibody was 5microg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076) and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice

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