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Secondary

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Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
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Western Blot of Donkey anti-Guinea Pig IgG Pre-Adsorbed Biotin Conjugated Secondary Antibody. Lane 1: Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.
Primary antibody: Biotin conjugated donkey anti-guinea pig IgG 1:1,000 for 60 min at RT.
Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Guinea Pig IgG. Other band(s): None.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
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Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
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Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
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ELISA Results of Purified Goat Anti-Golden Syrian Hamster IgG Antibody tested against purified Golden Syrian Hamster IgG. Each well was coated in duplicate with 1.0 µg of Golden Syrian Hamster IgG (p/n 007-0102). The working dilution is 1:183,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-067), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
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Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Dot Blot of Goat anti-Golden Syrian Hamster IgG Antibody Alkaline Phosphatase Conjugated. Antigen: Golden Syrian Hamster IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Primary antibody: n/a.
Secondary antibody: Goat anti-Golden Syrian Hamster IgG Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 1 HR at RT.
Block: MB-070 for 1 HR at RT.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHamster
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Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice

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