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Dot Blot of Goat anti-Guinea Pig IgG Fc Antibody Alkaline Phosphatase Conjugated. Antigen: Guinea Pig IgG Fc. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Primary antibody: none.
Secondary antibody: Goat anti-Guinea Pig IgG Fc Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT.
Block: MB-070 for 60 min at RT.
Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
ELISA Results of Goat Anti-Guinea Pig IgG Antibody Biotin Conjugated tested against purified Guinea Pig IgG Biotin. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 1:81,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Buffer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Donkey anti-Guinea Pig IgG Pre-Absorbed Rhodamine Conjugated Secondary Antibody. Lane 1: Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane.
Primary antibody: None.
Secondary antibody: Rhodamine donkey secondary antibody at 1:1,000 for 60 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Guinea Pig IgG. Other band(s): None.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
ELISA results of purified Donkey Anti-Guinea Pig IgG Antibody Min X10 Serums tested against purified Guinea Pig IgG. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Gt-a-Dky IgG HRP conjugate (p/n 616-1302), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
  • SizePrice
Western Blot of Donkey anti-Guinea Pig IgG Fluorescein Conjugated Antibody. Lane 1: Guinea Pig IgG. Lane 2: None. Load: 50 ng.
Primary antibody: None.
Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Guinea Pig IgG. Other band(s): None.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence
  • SizePrice
ELISA Results of Donkey Anti-Guinea Pig IgG mx10 Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 1:147,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMBE-1000 substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
  • SizePrice

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