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Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
ELISA results of purified Goat Anti-Mouse IgG Biotin Conjugated Antibody MinX 10 tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (red line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP, and TMB-1000 substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Dot Blot of Goat anti-Mouse IgG Antibody Phycoerythrin Conjugated. Antigen: Mouse IgG. Load: 3-fold serial dilution starting at 200 ng.
Primary antibody: None.
Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT.
Block: 5% BLOTTO 1 Hour at RT.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityMouse
  • SizePrice
Dot Blot of Goat anti-Mouse IgG2a Antibody Texas Red™ Conjugated Pre-absorbed. Antigen: Mouse IgG2a. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Primary antibody: n/a.
Secondary antibody: Goat anti-Mouse IgG2a Antibody Texas Red™ Conjugated Pre-absorbed at 1:1,000 for 1 HR at RT.
Block: MB-070 for 1 HR at RT.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Dot Blot of Goat anti-Mouse IgG2b Antibody Texas Red™ Conjugated Pre-absorbed. Antigen: Mouse IgG2b. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.
Primary antibody: n/a.
Secondary antibody: Goat anti-Mouse IgG2b Antibody Texas Red™ Conjugated Pre-absorbed at 1:1,000 for 1 HR at RT.
Block: MB-070 for 1 HR at RT.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Western Blot of Goat anti-Mouse IgG Texas Red Conjugated Antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane.
Primary antibody: None.
Secondary antibody: Texas Red goat secondary antibody at 1:1,000 for 60 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Mouse IgG. Other band(s): None.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityMouse
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Dot Blot of Mouse IgM (mu chain) (GOAT) Antibody. Lane 1A: Mouse IgM (100 ng).
Lane 1B: Mouse IgG (100 ng). Lanes 2-5: serial 3-fold dilution. Primary Antibody: Mouse IgM (mu chain) Antibody at 1 µg/mL at RT for 60 minutes.
Secondary Antibody: Donkey Anti-Goat IgG Antibody HRP Conj'd (p/n 605-703-125) at 1;40,000 dilution of 1 mg/mL at RT for 30 minutes.
Block: MB-070 at RT for 30 min.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
ELISA results of purified Goat anti-Mouse IgG Antibody Min X human serum tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG [red line], Human IgG [green line]. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Gt-anti-Donkey HRP conjugate 616-1302, and TMB-1000 substrate.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice

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