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Secondary

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Product group Antibodies
OriGene
Mouse IgG2a Antibody Pre-adsorbedTA397811
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
ELISA Results of Goat Anti-Mouse IgG2b (Gamma 2b chain) Antibody Minimum Cross Reactivity to Bovine, Human, and Rabbit Serum Proteins tested against purified Mouse IgG2b mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG2b (p/n 010-0142). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
Mouse IgG2b Antibody Pre-adsorbedTA397812
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
ELISA Results of Goat Anti-Mouse IgG3 (Gamma 3 chain) Antibody Minimum Cross Reactivity to Bovine, Human, and Rabbit Serum Proteins tested against purified Mouse IgG3 mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG3 (p/n 010-0143). The working dilution is 1:90,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Donkey Anti-Goat IgG HRP (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
Mouse IgG3 Antibody Pre-adsorbedTA397813
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
ELISA Results of Goat Anti-Mouse IgG (H&L) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins) tested against purified Mouse IgG mx10. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102) [Red Line], Bovine IgG (p/n 001-0102) [Green Line], Chicken IgG (p/n 003-0102) [Blue Line], Goat IgG (p/n 005-0102) [Purple Line], Guinea Pig IgG (p/n 006-0102), Hamster IgG (p/n 007-0102), Horse IgG (p/n 008-0102), Human IgG (p/n 009-0102), Rabbit IgG (p/n 011-0102), Rat IgG (p/n 012-0102), and Sheep IgG (p/n 013-0102). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).
Product group Antibodies
OriGene
Mouse IgG (H&L) Antibody Pre-adsorbedTA397814
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Product group Antibodies
OriGene
Mouse IgG IgA IgM (H&L) Pre-AdsorbedTA397815
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Western Blot of Goat anti-Mouse IgG Rhodamine Conjugated Antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Rhodamine goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Mouse IgG. Other band(s): None.
Product group Antibodies
OriGene
Mouse IgG (H&L) Antibody Rhodamine Conjugated Pre-AdsorbedTA397816
ApplicationsFlow Cytometry, ImmunoFluorescence
  • SizePrice
Western Blot of Fluorescein conjugated Goat anti-Mouse IgG1 (Gamma 1 chain) Pre-adsorbed secondary antibody. Lane 1: Mouse IgG1. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): none.
Product group Antibodies
OriGene
Mouse IgG1 Antibody Fluorescein Conjugated Pre-adsorbedTA397817
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Dot Blot of Goat anti-Mouse IgG2a Antibody Fluorescein Conjugated Pre-absorbed. Antigen: Mouse IgG2a. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Mouse IgG2a Antibody Fluorescein Conjugated Pre-absorbed at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.
Product group Antibodies
OriGene
Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbedTA397818
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Product group Antibodies
OriGene
Mouse IgG2b Antibody Fluorescein Conjugated Pre-adsorbedTA397819
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Product group Antibodies
OriGene
Mouse IgG3 Antibody Fluorescein Conjugated Pre-adsorbedTA397820
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Dot Blot showing the detection of Mouse IgG. A three-fold serial dilution of Mouse IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n B501-0500) 1 Hour at 20°C, Anti-Mouse IgG (H&L) (GOAT) Antibody Fluorescien Conjugated (p/n 610-102-121) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.
Product group Antibodies
OriGene
Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-AdsorbedTA397821
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityMouse
  • SizePrice
Product group Antibodies
OriGene
Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated Pre-AdsorbedTA397822
ApplicationsFlow Cytometry, ImmunoFluorescence
ReactivityMouse
  • SizePrice
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