![Untransfected (–) and transfected (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untransfected (–) and transfected (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637637/GTX637637_T-44837_20221230_WB_shRNA_watermark_23010400_431.webp)
Untransfected (–) and transfected (+) HeLa whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SEK1 / MKK4 antibody [HL1888]
GTX637637
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP2K4
Overview
- SupplierGeneTex
- Product NameSEK1 / MKK4 antibody [HL1888]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1888
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6416
- Target nameMAP2K4
- Target descriptionmitogen-activated protein kinase kinase 4
- Target synonymsJNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1, dual specificity mitogen-activated protein kinase kinase 4, JNK-activated kinase 1, JNK-activating kinase 1, MAP kinase kinase 4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, SAPK/ERK kinase 1, c-Jun N-terminal kinase kinase 1, c-Jun NH2-terminal kinase kinase 1, stress-activated protein kinase kinase 1
- HostRabbit
- IsotypeIgG
- Protein IDP45985
- Protein NameDual specificity mitogen-activated protein kinase kinase 4
- Scientific DescriptionThis gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637637/GTX637637_44970_20230317_WB_23032022_880.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637637/GTX637637_44970_20230317_WB_TPM_watermark_23032022_680.webp)
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637637/GTX637637_44970_20230421_WB_M_23042500_351.webp)
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637637/GTX637637_44970_20230421_WB_R_23042500_812.webp)
![SEK1 / MKK4 antibody [HL1888] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). SEK1 / MKK4 antibody [HL1888] detects SEK1 / MKK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEK1 / MKK4 stained by SEK1 / MKK4 antibody [HL1888] (GTX637637) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637637/GTX637637_44970_20230602_ICC_IF_23060622_782.webp)

![WB analysis of HepG2 (1), K562 (2), and HEK293 (3) cell lysate using GTX60399 SEK1 / MKK4 antibody [5H4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60399/GTX60399_20170912_WB_w_23061123_426.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with SEK1 / MKK4 antibody [HL1839] (GTX637564) diluted at 1:5000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637564/GTX637564_T-44823_20230414_WB_multiple_B_23041723_957.webp)

![ICC/IF analysis of COS7 cells transiently transfected with SEK1 / MKK4 plasmid using GTX84161 SEK1 / MKK4 antibody [4B4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84161/GTX84161_970_ICCIF_w_23061420_186.webp)
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84162 SEK1 / MKK4 antibody [8A8]. Loading : 10 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84162/GTX84162_4449_WB_w_23061420_553.webp)
