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ProductsBack/SEK1/MKK4 (phospho-Ser257/Thr261) rabbit pAb

SEK1/MKK4 (phospho-Ser257/Thr261) rabbit pAb

ORB2303887
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Biorbyt
  • Product Name
    SEK1/MKK4 (phospho-Ser257/Thr261) rabbit pAb
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Isotype
    IgG
  • Scientific Description
    SEK1/MKK4 (phospho-Ser257/Thr261) rabbit pAb
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAP2K4 using anti-MAP2K4 antibody (A01725-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K4 antigen affinity purified polyclonal antibody (Catalog # A01725-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2K4 at approximately 44KD. The expected band size for MAP2K4 is at 44KD.
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