Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (GTX640900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (GTX640900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640900/GTX640900_T-45523_20240906_WB_M_tissue_24091102_950.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (GTX640900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (GTX640900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640900/GTX640900_T-45523_20240906_WB_R_liver_24091102_487.webp "Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (GTX640900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Seladin 1 antibody [HL3253] detects Seladin 1 protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Seladin 1 stained by Seladin 1 antibody [HL3253] (GTX640900) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640900/GTX640900_T-45523_20240920_ICC_IF_24092600_795.webp "Seladin 1 antibody [HL3253] detects Seladin 1 protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Seladin 1 stained by Seladin 1 antibody [HL3253] (GTX640900) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (GTX640900) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Seladin 1 antibody [HL3253]
GTX640900
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDHCR24
Overview
- SupplierGeneTex
- Product NameSeladin 1 antibody [HL3253]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL3253
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1718
- Target nameDHCR24
- Target description24-dehydrocholesterol reductase
- Target synonymsDCE, Nbla03646, SELADIN1, seladin-1, delta(24)-sterol reductase, 3 beta-hydroxysterol delta 24-reductase, 3beta-hydroxysterol delta24 reductase, desmosterol-to-cholesterol enzyme, diminuto/dwarf1 homolog, seladin 1, selective AD indicator 1
- HostRabbit
- IsotypeIgG
- Protein IDQ15392
- Protein NameDelta(24)-sterol reductase
- Scientific DescriptionThis gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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