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SEMA3C Antibody

CSB-PA006602
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEMA3C
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEMA3C Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10512
  • Target name
    SEMA3C
  • Target description
    semaphorin 3C
  • Target synonyms
    sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C; sema E; SEMAE; semaphorin E; semaphorin-3C; SemE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99985
  • Protein Name
    Semaphorin-3C
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEMA3C using anti-SEMA3C antibody (A06444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissues lysates Lane 2: human A549 whole cell lysates Lane 3: human U2OS whole cell lysates Lane 4: rat liver tissue lysates Lane 5: mouse lung tissue lysates Lane 6: mouse kidney tissue lysates Lane 7: mouse liver tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3C antigen affinity purified polyclonal antibody (Catalog # A06444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3C at approximately 85KD. The expected band size for SEMA3C is at 85KD.
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