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Western blot analysis of extracts from Jurkat cells, using SEPT1 antibody.
Western blot analysis of extracts from Jurkat cells, using SEPT1 antibody.
Western blot analysis of extracts from Jurkat cells, using SEPT1 antibody.

SEPT1 Antibody

CSB-PA270288
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEPTIN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEPT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1731
  • Target name
    SEPTIN1
  • Target description
    septin 1
  • Target synonyms
    DIFF6, LARP, PNUTL3, SEP1, SEPT1, Septin-1, septin-1, differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase), peanut-like protein 3, serologically defined breast cancer antigen NY-BR-24
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WYJ6
  • Protein Name
    Septin-1
  • Scientific Description
    Filament-forming cytoskeletal GTPase By similarity. May play a role in cytokinesis Potential. Tu Q., Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases. The MGC Project Team, Genome Res. 14:2121-2127(2004). Scanlan M.J., Cancer Immun. 1:4-4(2001).
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of LARP/SEPTIN1 using anti-LARP/SEPTIN1 antibody (A30537-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse EL-4 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARP/SEPTIN1 antigen affinity purified polyclonal antibody (Catalog # A30537-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARP/SEPTIN1 at approximately 40 kDa. The expected band size for LARP/SEPTIN1 is at 40 kDa.
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