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WB analysis of Jurkat lysate using GTX89243 SEPT1 antibody, Internal. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of Jurkat lysate using GTX89243 SEPT1 antibody, Internal. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of Jurkat lysate using GTX89243 SEPT1 antibody, Internal. Dilution : 2microg/ml Loading : 35microg protein in RIPA buffer

SEPT1 antibody, Internal

GTX89243
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSEPTIN1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SEPT1 antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 2-4microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1731
  • Target name
    SEPTIN1
  • Target description
    septin 1
  • Target synonyms
    DIFF6; differentiation 6 (deoxyguanosine triphosphate triphosphohydrolase); LARP; peanut-like protein 3; PNUTL3; SEP1; SEPT1; septin-1; serologically defined breast cancer antigen NY-BR-24
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ8WYJ6
  • Protein Name
    Septin-1
  • Scientific Description
    This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimers disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LARP/SEPTIN1 using anti-LARP/SEPTIN1 antibody (A30537-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse EL-4 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARP/SEPTIN1 antigen affinity purified polyclonal antibody (Catalog # A30537-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LARP/SEPTIN1 at approximately 40 kDa. The expected band size for LARP/SEPTIN1 is at 40 kDa.
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