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ProductsBack/SEPT12 / Septin 12 Antibody (Biotin)

SEPT12 / Septin 12 Antibody (Biotin)

LS-C399927
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetSEPTIN12
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SEPT12 / Septin 12 Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID124404
  • Target name
    SEPTIN12
  • Target description
    septin 12
  • Target synonyms
    SEPT12, SPGF10, septin-12, testicular tissue protein Li 168
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEPTIN12 using anti-SEPTIN12 antibody (A12342-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPTIN12 antigen affinity purified polyclonal antibody (Catalog # A12342-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEPTIN12 at approximately 45 kDa. The expected band size for SEPTIN12 is at 41 kDa.
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