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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA286864(SEPT9 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA286864(SEPT9 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA286864(SEPT9 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)

SEPT9 Antibody

CSB-PA286864
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEPTIN9
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEPT9 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10801
  • Target name
    SEPTIN9
  • Target description
    septin 9
  • Target synonyms
    AF17q25, MSF, MSF1, NAPB, PNUTL4, SEPT9, SINT1, SeptD1, septin-9, MLL septin-like fusion protein MSF-A, Ov/Br septin, ovarian/breast septin, peanut-like 4, septin D1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHD8
  • Protein Name
    Septin-9
  • Scientific Description
    This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MSF/SEPTIN9 using anti-MSF/SEPTIN9 antibody (A05279-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: human SH-SY5Y whole cell lsates, Lane 8: huamn PC-3 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSF/SEPTIN9 antigen affinity purified polyclonal antibody (Catalog # A05279-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSF/SEPTIN9 at approximately 70 kDa. The expected band size for MSF/SEPTIN9 is at 65 kDa.
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