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ProductsBack/SEPT9 / Septin 9 Antibody

SEPT9 / Septin 9 Antibody

LS-C668614
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSEPTIN9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SEPT9 / Septin 9 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of Septin 9 expression in Jurkat (A), Hela (B) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of Septin 9 expression in Jurkat (A), Hela (B) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10801
  • Target name
    SEPTIN9
  • Target description
    septin 9
  • Target synonyms
    AF17q25, MSF, MSF1, NAPB, PNUTL4, SEPT9, SINT1, SeptD1, septin-9, MLL septin-like fusion protein MSF-A, Ov/Br septin, ovarian/breast septin, peanut-like 4, septin D1
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MSF/SEPTIN9 using anti-MSF/SEPTIN9 antibody (A05279-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: human SH-SY5Y whole cell lsates, Lane 8: huamn PC-3 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSF/SEPTIN9 antigen affinity purified polyclonal antibody (Catalog # A05279-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSF/SEPTIN9 at approximately 70 kDa. The expected band size for MSF/SEPTIN9 is at 65 kDa.
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