IHC-P analysis of human liver tissue using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
![IHC-P analysis of human skeletal muscle tissue using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22861/GTX22861_1176_IHC-P_w_23060620_258.webp "IHC-P analysis of human skeletal muscle tissue using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22861/GTX22861_1177_IHC-P_w_23060620_227.webp "IHC-P analysis of human tonsil tissue using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![WB analysis of whole cell extracts (30 microg lysate) of DU-145 (Lane 1), L6 (Lane 2), U-87MG (Lane 3) and PANC 1 (Lane 4) using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22861/GTX22861_1616_WB_w_23060620_906.webp "WB analysis of whole cell extracts (30 microg lysate) of DU-145 (Lane 1), L6 (Lane 2), U-87MG (Lane 3) and PANC 1 (Lane 4) using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Dilution : 1:1000")
![ICC/IF analysis of A549 cells using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22861/GTX22861_522_ICC-IF_w_23060620_909.webp "ICC/IF analysis of A549 cells using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22861/GTX22861_523_ICC-IF_w_23060620_889.webp "ICC/IF analysis of HeLa cells using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of U251 cells using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22861/GTX22861_524_ICC-IF_w_23060620_437.webp "ICC/IF analysis of U251 cells using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human liver tissue using GTX22861 SERCA2 ATPase antibody [2A7-A1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
SERCA2 ATPase antibody [2A7-A1]
GTX22861
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAmphibian, Canine, Drosophila, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rodent, Rat, Sheep, Xenopus
TargetATP2A2
Overview
- SupplierGeneTex
- Product NameSERCA2 ATPase antibody [2A7-A1]
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes the SERCA2a and SERCA2b isoforms identically. However, the antibody does not recognize the skeletal muscle (SERCA1) or housekeeping isoforms (SERCA3).
- Application Supplier NoteWB: 1:1,000. ICC/IF: 1:100-1:250. IHC-P: 1 microg/ml. IHC-Fr: 1:100. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A7-A1
- ConjugateUnconjugated
- Gene ID403878
- Target nameATP2A2
- Target descriptionATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
- Target synonymscalcium ATPase; calcium pump 2; endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2; SERCA2A; SR Ca(2+)-ATPase 2
- HostMouse
- IsotypeIgG2a
- Protein IDO46674
- Protein NameSarcoplasmic/endoplasmic reticulum calcium ATPase 2
- ReactivityAmphibian, Canine, Drosophila, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rodent, Rat, Sheep, Xenopus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Targeted disruption of PDE3B, but not PDE3A, protects murine heart from ischemia/reperfusion injury. Chung YW et al., 2015 Apr 28, Proc Natl Acad Sci U S ARead more