IHC-P analysis of human heart tissue using GTX22817 SERCA2 ATPase antibody [IID8]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of human liver tissue using GTX22817 SERCA2 ATPase antibody [IID8]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22817/GTX22817_1153_IHC-P_w_23060620_350.webp "IHC-P analysis of human liver tissue using GTX22817 SERCA2 ATPase antibody [IID8]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX22817 SERCA2 ATPase antibody [IID8]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22817/GTX22817_1154_IHC-P_w_23060620_773.webp "IHC-P analysis of human tonsil tissue using GTX22817 SERCA2 ATPase antibody [IID8]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![WB analysis of 25 ug of rat skeletal muscle (lane 1) and human skeletal muscle (lane 2) using GTX22817 SERCA2 ATPase antibody [IID8]. Dilution : 1:1000 (rat muscle) and 1:2000 (human muscle)](https://www.genetex.com/upload/website/prouct_img/normal/GTX22817/GTX22817_1607_WB_w_23060620_875.webp "WB analysis of 25 ug of rat skeletal muscle (lane 1) and human skeletal muscle (lane 2) using GTX22817 SERCA2 ATPase antibody [IID8]. Dilution : 1:1000 (rat muscle) and 1:2000 (human muscle)")
![ICC/IF analysis of A549 cells using GTX22817 SERCA2 ATPase antibody [IID8]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22817/GTX22817_501_ICC-IF_w_23060620_502.webp "ICC/IF analysis of A549 cells using GTX22817 SERCA2 ATPase antibody [IID8]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX22817 SERCA2 ATPase antibody [IID8]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22817/GTX22817_502_ICC-IF_w_23060620_778.webp "ICC/IF analysis of HeLa cells using GTX22817 SERCA2 ATPase antibody [IID8]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of U251 cells using GTX22817 SERCA2 ATPase antibody [IID8]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22817/GTX22817_503_ICC-IF_w_23060620_729.webp "ICC/IF analysis of U251 cells using GTX22817 SERCA2 ATPase antibody [IID8]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human heart tissue using GTX22817 SERCA2 ATPase antibody [IID8]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
SERCA2 ATPase antibody [IID8]
GTX22817
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityAmphibian, Bovine, Canine, Guinea Pig, Hamster, Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep
TargetATP2A2
Overview
- SupplierGeneTex
- Product NameSERCA2 ATPase antibody [IID8]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects sarcoplasmic or endoplasmic reticulum calcium 2 (SERCA2) ATPase.
- Application Supplier NoteWB: 1:500-1:5000. ICC/IF: 1:100-1:200. IHC-P: 1 microg/ml. IHC-Fr: 1:500. FACS: 1microg per 106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDIID8
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID403878
- Target nameATP2A2
- Target descriptionATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
- Target synonymscalcium ATPase; calcium pump 2; endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2; SERCA2A; SR Ca(2+)-ATPase 2
- HostMouse
- IsotypeIgG1
- Protein IDO46674
- Protein NameSarcoplasmic/endoplasmic reticulum calcium ATPase 2
- ReactivityAmphibian, Bovine, Canine, Guinea Pig, Hamster, Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203