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Immunohistochemistry of paraffin-embedded Human lung cancer tissue using SERPINA3 Polyclonal Antibody at dilution 1:55
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using SERPINA3 Polyclonal Antibody at dilution 1:55
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using SERPINA3 Polyclonal Antibody at dilution 1:55

SERPINA3 Polyclonal Antibody

E-AB-10777
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SERPINA3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this proteins sequence have been implicated in Alzheimers disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AACT/SERPINA3 using anti-AACT/SERPINA3 antibody (A02312-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: human HUH-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AACT/SERPINA3 antigen affinity purified polyclonal antibody (Catalog # A02312-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AACT/SERPINA3 at approximately 50 kDa. The expected band size for AACT/SERPINA3 is at 48 kDa.
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