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Western Blot analysis of K562 cells using Kallistatin Polyclonal Antibody
Western Blot analysis of K562 cells using Kallistatin Polyclonal Antibody
Western Blot analysis of K562 cells using Kallistatin Polyclonal Antibody

SERPINA4 Antibody

CSB-PA005146
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSERPINA4
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Overview

  • Supplier
    Cusabio
  • Product Name
    SERPINA4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5267
  • Target name
    SERPINA4
  • Target description
    serpin family A member 4
  • Target synonyms
    KAL, KLST, KST, PI-4, PI4, kallistatin, kallistatin, kallikrein inhibitor, peptidase inhibitor 4, protease inhibitor 4 (kallistatin), serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, serpin A4, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4, sserpin family A member 4, testicular secretory protein Li 22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29622
  • Protein Name
    Kallistatin
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SERPINA4 using anti-SERPINA4 antibody (A08426). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA4 antigen affinity purified polyclonal antibody (Catalog # A08426) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA4 at approximately 49KD. The expected band size for SERPINA4 is at 49KD.
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