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Western blot All lanes: Leukocyte elastase inhibitor antibody at 4ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 27 kDa Observed band size: 43 kDa
Western blot All lanes: Leukocyte elastase inhibitor antibody at 4ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 27 kDa Observed band size: 43 kDa
Western blot All lanes: Leukocyte elastase inhibitor antibody at 4ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 27 kDa Observed band size: 43 kDa

SERPINB1 Antibody

CSB-PA021065ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSERPINB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SERPINB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1992
  • Target name
    SERPINB1
  • Target description
    serpin family B member 1
  • Target synonyms
    EI, ELANH2, HEL-S-27, HEL57, LEI, M/NEI, MNEI, PI-2, PI2, leukocyte elastase inhibitor, epididymis luminal protein 57, epididymis secretory protein Li 27, peptidase inhibitor 2, protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1, serpin peptidase inhibitor, clade B (ovalbumin), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30740
  • Protein Name
    Leukocyte elastase inhibitor
  • Scientific Description
    Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Also functions as a potent intracellular inhibitor of granzyme H.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PI2/SERPINB1 using anti-PI2/SERPINB1 antibody (A04491-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI2/SERPINB1 antigen affinity purified polyclonal antibody (Catalog # A04491-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI2/SERPINB1 at approximately 43 kDa. The expected band size for PI2/SERPINB1 is at 43 kDa.
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