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SERPINB1 Antibody

LS-C334593
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSERPINB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SERPINB1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 26kDa/42kDa, while the observed MW by Western blot was 43kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:20 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 26kDa/42kDa, while the observed MW by Western blot was 43kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.18 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1992
  • Target name
    SERPINB1
  • Target description
    serpin family B member 1
  • Target synonyms
    EI; ELANH2; epididymis luminal protein 57; epididymis secretory protein Li 27; HEL57; HEL-S-27; LEI; leukocyte elastase inhibitor; M/NEI; MNEI; peptidase inhibitor 2; PI2; PI-2; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1; serpin peptidase inhibitor, clade B (ovalbumin), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PI2/SERPINB1 using anti-PI2/SERPINB1 antibody (A04491-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI2/SERPINB1 antigen affinity purified polyclonal antibody (Catalog # A04491-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI2/SERPINB1 at approximately 43 kDa. The expected band size for PI2/SERPINB1 is at 43 kDa.
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