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SERPINB13 antibody

GTX70462
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetSERPINB13
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Overview

  • Supplier
    GeneTex
  • Product Name
    SERPINB13 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5275
  • Target name
    SERPINB13
  • Target description
    serpin family B member 13
  • Target synonyms
    haCaT UV-repressible serpin; headpin; HSHUR7SEQ; HUR7; HURPIN; peptidase inhibitor 13; PI13; PI-13; protease inhibitor 13 (hurpin, headpin); proteinase inhibitor 13; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 13; serpin B13; serpin peptidase inhibitor, clade B (ovalbumin), member 13; UV-B repressed sequence, HUR 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UIV8
  • Protein Name
    Serpin B13
  • Scientific Description
    The protein encoded by this gene is a member of the serpin family of serine protease inhibitors. The encoded protein inhibits the activity of cathepsin K and is itself transcriptionally repressed by RUNX1. This gene is downregulated in many types of cancer. [provided by RefSeq, Jan 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERPINB13 using anti-SERPINB13 antibody (A09602-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB13 antigen affinity purified polyclonal antibody (Catalog # A09602-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB13 at approximately 44 kDa. The expected band size for SERPINB13 is at 44 kDa.
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