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ProductsBack/SERPINB13 / HUR7 Antibody

SERPINB13 / HUR7 Antibody

LS-C748779
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSERPINB13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SERPINB13 / HUR7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 38kDa/44kDa, while the observed MW by Western blot was 44kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 38kDa/44kDa, while the observed MW by Western blot was 44kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5275
  • Target name
    SERPINB13
  • Target description
    serpin family B member 13
  • Target synonyms
    HSHUR7SEQ, HUR7, PI13, headpin, serpin B13, HURPIN, PI-13, UV-B repressed sequence, HUR 7, haCaT UV-repressible serpin, peptidase inhibitor 13, protease inhibitor 13 (hurpin, headpin), proteinase inhibitor 13, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 13, serpin peptidase inhibitor, clade B (ovalbumin), member 13
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERPINB13 using anti-SERPINB13 antibody (A09602-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB13 antigen affinity purified polyclonal antibody (Catalog # A09602-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB13 at approximately 44 kDa. The expected band size for SERPINB13 is at 44 kDa.
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