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ProductsBack/SERPINB13 / HUR7 Antibody (clone OTI2B6)

SERPINB13 / HUR7 Antibody (clone OTI2B6)

LS-C173372
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSERPINB13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SERPINB13 / HUR7 Antibody (clone OTI2B6)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:150), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2B6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5275
  • Target name
    SERPINB13
  • Target description
    serpin family B member 13
  • Target synonyms
    haCaT UV-repressible serpin; headpin; HSHUR7SEQ; HUR7; HURPIN; peptidase inhibitor 13; PI13; PI-13; protease inhibitor 13 (hurpin, headpin); proteinase inhibitor 13; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 13; serpin B13; serpin peptidase inhibitor, clade B (ovalbumin), member 13; UV-B repressed sequence, HUR 7
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERPINB13 using anti-SERPINB13 antibody (A09602-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB13 antigen affinity purified polyclonal antibody (Catalog # A09602-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB13 at approximately 44 kDa. The expected band size for SERPINB13 is at 44 kDa.
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