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SerpinB8 (PI-8) Alexa Fluor(r) 488

SC-101371AF488
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSERPINB8
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    SerpinB8 (PI-8) Alexa Fluor(r) 488
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 488
  • Gene ID5271
  • Target name
    SERPINB8
  • Target description
    serpin family B member 8
  • Target synonyms
    C18orf53, CAP2, PI-8, PI8, PSS5, serpin B8, cytoplasmic antiproteinase 2, peptidase inhibitor 8, protease inhibitor 8 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 8, serpin peptidase inhibitor, clade B (ovalbumin), member 8
  • Host
    Mouse
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERPINB8 using anti-SERPINB8 antibody (A11265-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB8 antigen affinity purified polyclonal antibody (Catalog # A11265-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB8 at approximately 48 kDa. The expected band size for SERPINB8 is at 43 kDa.
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