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Western Blot analysis of Jurkat cells using PI-9 Polyclonal Antibody
Western Blot analysis of Jurkat cells using PI-9 Polyclonal Antibody
Western Blot analysis of Jurkat cells using PI-9 Polyclonal Antibody

SERPINB9 Antibody

CSB-PA003772
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSERPINB9
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Overview

  • Supplier
    Cusabio
  • Product Name
    SERPINB9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5272
  • Target name
    SERPINB9
  • Target description
    serpin family B member 9
  • Target synonyms
    CAP3; CAP-3; cytoplasmic antiproteinase 3; peptidase inhibitor 9; PI9; PI-9; protease inhibitor 9 (ovalbumin type); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9; serpin B9; serpin peptidase inhibitor, clade B (ovalbumin), member 9; serpin peptidase inhibitor, clade B, member 9; testicular tissue protein Li 180
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50453
  • Protein Name
    Serpin B9
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SERPINB9 using anti-SERPINB9 antibody (A04734-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB9 antigen affinity purified polyclonal antibody (Catalog # A04734-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB9 at approximately 38 kDa. The expected band size for SERPINB9 is at 38,42 kDa.
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