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WB analysis of various sample lysates using GTX54693 SerpinB9 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54693 SerpinB9 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54693 SerpinB9 antibody. Dilution : 1:1000 Loading : 25microg per lane

SerpinB9 antibody

GTX54693
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetSERPINB9
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Overview

  • Supplier
    GeneTex
  • Product Name
    SerpinB9 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:10 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5272
  • Target name
    SERPINB9
  • Target description
    serpin family B member 9
  • Target synonyms
    CAP3; CAP-3; cytoplasmic antiproteinase 3; peptidase inhibitor 9; PI9; PI-9; protease inhibitor 9 (ovalbumin type); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9; serpin B9; serpin peptidase inhibitor, clade B (ovalbumin), member 9; serpin peptidase inhibitor, clade B, member 9; testicular tissue protein Li 180
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50453
  • Protein Name
    Serpin B9
  • Scientific Description
    This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERPINB9 using anti-SERPINB9 antibody (A04734-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB9 antigen affinity purified polyclonal antibody (Catalog # A04734-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB9 at approximately 38 kDa. The expected band size for SERPINB9 is at 38,42 kDa.
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