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ProductsBack/SERPINH1 Antibody
Western blot All lanes: Serpin H1 antibody at 2ug/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: A2780 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa
Western blot All lanes: Serpin H1 antibody at 2ug/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: A2780 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa
Western blot All lanes: Serpin H1 antibody at 2ug/ml Lane 1: NIH/3T3 whole cell lysate Lane 2: A2780 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa

SERPINH1 Antibody

CSB-PA021087ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSERPINH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SERPINH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID871
  • Target name
    SERPINH1
  • Target description
    serpin family H member 1
  • Target synonyms
    AsTP3, CBP1, CBP2, HSP47, OI10, PIG14, PPROM, RA-A47, SERPINH2, gp46, serpin H1, 47 kDa heat shock protein, arsenic-transactivated protein 3, cell proliferation-inducing gene 14 protein, collagen binding protein 1, colligin-1, colligin-2, heat shock protein 47, rheumatoid arthritis antigen A-47, rheumatoid arthritis-related antigen RA-A47, serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1), serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2), serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50454
  • Protein Name
    Serpin H1
  • Scientific Description
    Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody (PB9325). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human SK-N-SH whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat L6 whole cell lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp47/SERPINH1 antigen affinity purified polyclonal antibody (Catalog # PB9325) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp47/SERPINH1 at approximately 46 kDa. The expected band size for Hsp47/SERPINH1 is at 46 kDa.
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