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SETD3 Antibody

LS-C409610
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSETD3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SETD3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 32kDa/33kDa/67kDa, while the observed MW by Western blot was 70kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 32kDa/33kDa/67kDa, while the observed MW by Western blot was 70kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84193
  • Target name
    SETD3
  • Target description
    SET domain containing 3, actin histidine methyltransferase
  • Target synonyms
    actin-histidine N-methyltransferase; C14orf154; histone-lysine N-methyltransferase setd3; hSETD3; protein-L-histidine N-tele-methyltransferase; SET domain-containing protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SETD3 using anti-SETD3 antibody (A10965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETD3 antigen affinity purified polyclonal antibody (Catalog # A10965-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SETD3 at approximately 60 kDa. The expected band size for SETD3 is at 67 kDa.
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IHC image of CSB-PA769772LA01HU diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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