![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115305/GTX115305_40604_20230224_WB_23030219_547.webp)
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SETDB1 antibody [N1], N-term
GTX115305
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSETDB1
Overview
- SupplierGeneTex
- Product NameSETDB1 antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.53 mg/ml
- ConjugateUnconjugated
- Gene ID9869
- Target nameSETDB1
- Target descriptionSET domain bifurcated histone lysine methyltransferase 1
- Target synonymsESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21, histone-lysine N-methyltransferase SETDB1, ERG-associated protein with a SET domain, ESET, SET domain bifurcated 1, histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase, H3lysine-9 specific 4, lysine N-methyltransferase 1E, tudor domain containing 21
- HostRabbit
- IsotypeIgG
- Protein IDQ15047
- Protein NameHistone-lysine N-methyltransferase SETDB1
- Scientific DescriptionThis gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wu K, Liu H, Wang Y, et al. SETDB1-Mediated Cell Fate Transition between 2C-Like and Pluripotent States. Cell Rep. 2020,30(1):25-36.e6. doi: 10.1016/j.celrep.2019.12.010Read this paper
- Ho YJ, Lin YM, Huang YC, et al. Significance of histone methyltransferase SETDB1 expression in colon adenocarcinoma. APMIS. 2017,125(11):985-995. doi: 10.1111/apm.12745Read this paper
- Chen J, Liu H, Liu J, et al. H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs. Nat Genet. 2013,45(1):34-42. doi: 10.1038/ng.2491Read this paper
![SETDB1 antibody [N1], N-term immunoprecipitates SETDB1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 μg SETDB1 antibody [N1], N-term (GTX115305) 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent] SETDB1 antibody [N1], N-term immunoprecipitates SETDB1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 μg SETDB1 antibody [N1], N-term (GTX115305) 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX115305/GTX115305_40604_IP_w_23060519_814.webp)

![HeLa whole cell and nuclear extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. HeLa whole cell and nuclear extract (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115305/GTX115305_45301_20240329_WB_Fraction_24090423_384.webp)

![SETDB1 antibody [N2C1], Internal immunoprecipitates SETDB1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 μg SETDB1 antibody [N2C1], Internal (GTX110219) 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX110219/GTX110219_40471_IP_w_23060500_420.webp)
![Various tissue extracts (50 Aμg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637226/GTX637226_T-44746_20220805_WB_M_R_22080923_527.webp)



![Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3259] (GTX640906) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640906/GTX640906_T-45523_20240913_WB_M_testis_24091901_114.webp)