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Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SETDB1 antibody [N1], N-term

GTX115305
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSETDB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SETDB1 antibody [N1], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.53 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9869
  • Target name
    SETDB1
  • Target description
    SET domain bifurcated histone lysine methyltransferase 1
  • Target synonyms
    ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21, histone-lysine N-methyltransferase SETDB1, ERG-associated protein with a SET domain, ESET, SET domain bifurcated 1, histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase, H3lysine-9 specific 4, lysine N-methyltransferase 1E, tudor domain containing 21
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15047
  • Protein Name
    Histone-lysine N-methyltransferase SETDB1
  • Scientific Description
    This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wu K, Liu H, Wang Y, et al. SETDB1-Mediated Cell Fate Transition between 2C-Like and Pluripotent States. Cell Rep. 2020,30(1):25-36.e6. doi: 10.1016/j.celrep.2019.12.010
    Read this paper
  • Ho YJ, Lin YM, Huang YC, et al. Significance of histone methyltransferase SETDB1 expression in colon adenocarcinoma. APMIS. 2017,125(11):985-995. doi: 10.1111/apm.12745
    Read this paper
  • Chen J, Liu H, Liu J, et al. H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs. Nat Genet. 2013,45(1):34-42. doi: 10.1038/ng.2491
    Read this paper