SETDB1 antibody [N2C1], Internal immunoprecipitates SETDB1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 μg SETDB1 antibody [N2C1], Internal (GTX110219) 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
![SETDB1 antibody [N2C1], Internal detects SETDB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB1 stained by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110219/GTX110219_44504_20220408_ICC_IF_w_23060500_884.webp "SETDB1 antibody [N2C1], Internal detects SETDB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB1 stained by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Scale bar= 10μm.")
![SETDB1 antibody [N2C1], Internal detects SETDB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. SETDB1 stained by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110219/GTX110219_44503_20220114_IHC-P_M_w_23060500_407.webp "SETDB1 antibody [N2C1], Internal detects SETDB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. SETDB1 stained by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110219/GTX110219_40471_20210813_WB_Fraction_w_23060500_112.webp "HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
SETDB1 antibody [N2C1], Internal immunoprecipitates SETDB1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 μg SETDB1 antibody [N2C1], Internal (GTX110219) 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N2C1], Internal (GTX110219) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
SETDB1 antibody [N2C1], Internal
GTX110219
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSETDB1
Overview
- SupplierGeneTex
- Product NameSETDB1 antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID9869
- Target nameSETDB1
- Target descriptionSET domain bifurcated histone lysine methyltransferase 1
- Target synonymsESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21, histone-lysine N-methyltransferase SETDB1, ERG-associated protein with a SET domain, ESET, SET domain bifurcated 1, histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase, H3lysine-9 specific 4, lysine N-methyltransferase 1E, tudor domain containing 21
- HostRabbit
- IsotypeIgG
- Protein IDQ15047
- Protein NameHistone-lysine N-methyltransferase SETDB1
- Scientific DescriptionThis gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Li Y, Wang Y, Gao L, et al. Betulinic acid self-assembled nanoparticles for effective treatment of glioblastoma. J Nanobiotechnology. 2022,20(1):39. doi: 10.1186/s12951-022-01238-7Read this paper
Datasheet
Related products
Product group Antibodies
Anti-SETDB1 [RAB-C531]AB01865-1.1-BT
ApplicationsImmunoFluorescence, ImmunoPrecipitation
ReactivityHuman
TargetSETDB1
- SizePrice
Product group Antibodies
Anti-SETDB1 Antibody144-06145
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSETDB1
- SizePrice
![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115305/GTX115305_40604_20230224_WB_23030219_547.webp)
Product group Antibodies
References
SETDB1 antibody [N1], N-termGTX115305
ApplicationsImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetSETDB1
- SizePrice
![Various tissue extracts (50 Aμg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637226/GTX637226_T-44746_20220805_WB_M_R_22080923_527.webp)
Product group Antibodies
SETDB1 antibody [HL1645]GTX637226
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetSETDB1
- SizePrice
Product group Antibodies
Anti-SETDB1 AntibodyA31125
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
- SizePrice
Product group Antibodies
SETDB1 Recombinant AntibodyBSM-60316M
ApplicationsWestern Blot
ReactivityHuman, Monkey, Mouse, Rat
TargetSETDB1
- SizePrice
Product group Antibodies
SETDB1 antibodyGTX33492
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetSETDB1
- SizePrice
![Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL3259] (GTX640906) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640906/GTX640906_T-45523_20240913_WB_M_testis_24091901_114.webp)
Product group Antibodies
SETDB1 antibody [HL3259]GTX640906
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetSETDB1
- SizePrice