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Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 5% SDS PAGE GTX112116 diluted at 1:10000
Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 5% SDS PAGE GTX112116 diluted at 1:10000
Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 5% SDS PAGE GTX112116 diluted at 1:10000

SFPQ antibody

GTX112116
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSFPQ
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Overview

  • Supplier
    GeneTex
  • Product Name
    SFPQ antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6421
  • Target name
    SFPQ
  • Target description
    splicing factor proline and glutamine rich
  • Target synonyms
    POMP100, PPP1R140, PSF, splicing factor, proline- and glutamine-rich, 100 kDa DNA-pairing protein, DNA-binding p52/p100 complex, 100 kDa subunit, PTB-associated splicing factor, epididymis secretory sperm binding protein, polypyrimidine tract binding protein associated, polypyrimidine tract-binding protein-associated splicing factor, protein phosphatase 1, regulatory subunit 140, splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated), splicing factor proline/glutamine-rich
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23246
  • Protein Name
    Splicing factor, proline- and glutamine-rich
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012
    Read this paper

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Figure 1. Western blot analysis of SFPQ using anti-SFPQ antibody (A02243-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse C2C12 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFPQ antigen affinity purified polyclonal antibody (Catalog # A02243-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFPQ at approximately 100 kDa. The expected band size for SFPQ is at 76 kDa.
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