SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114209/GTX114209_44398_20210813_WB_w_23060501_919.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX114209/GTX114209_40828_20160127_IHC-P_M_w_23060501_221.webp "SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![SFPQ antibody [N2C1], Internal detects SFPQ protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114209/GTX114209_41927_20160113_IFA_w_23060501_635.webp "SFPQ antibody [N2C1], Internal detects SFPQ protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.")
![Immunoprecipitation of SFPQ protein from 293T whole cell extracts using 5 μg of SFPQ antibody [N2C1], Internal (GTX114209). Western blot analysis was performed using SFPQ antibody [N2C1], Internal (GTX114209). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX114209/GTX114209_40828_20150618_IP_w_23060501_991.webp "Immunoprecipitation of SFPQ protein from 293T whole cell extracts using 5 μg of SFPQ antibody [N2C1], Internal (GTX114209). Western blot analysis was performed using SFPQ antibody [N2C1], Internal (GTX114209). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SFPQ antibody [N2C1], Internal
GTX114209
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSFPQ
Overview
- SupplierGeneTex
- Product NameSFPQ antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.78 mg/ml
- ConjugateUnconjugated
- Gene ID6421
- Target nameSFPQ
- Target descriptionsplicing factor proline and glutamine rich
- Target synonymsPOMP100, PPP1R140, PSF, splicing factor, proline- and glutamine-rich, 100 kDa DNA-pairing protein, DNA-binding p52/p100 complex, 100 kDa subunit, PTB-associated splicing factor, epididymis secretory sperm binding protein, polypyrimidine tract binding protein associated, polypyrimidine tract-binding protein-associated splicing factor, protein phosphatase 1, regulatory subunit 140, splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated), splicing factor proline/glutamine-rich
- HostRabbit
- IsotypeIgG
- Protein IDP23246
- Protein NameSplicing factor, proline- and glutamine-rich
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Gao Z, Chen M, Tian X, et al. A novel human lncRNA SANT1 cis-regulates the expression of SLC47A2 by altering SFPQ/E2F1/HDAC1 binding to the promoter region in renal cell carcinoma. RNA Biol. 2019,16(7):940-949. doi: 10.1080/15476286.2019.1602436Read this paper
- Raab JR, Smith KN, Spear CC, et al. SWI/SNF remains localized to chromatin in the presence of SCHLAP1. Nat Genet. 2019,51(1):26-29. doi: 10.1038/s41588-018-0272-zRead this paper
Datasheet
Related products
Product group Antibodies
Anti-SFPQ Antibody144-00958
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSFPQ
- SizePrice
Product group Antibodies
Anti-SFPQ Antibody Picoband(r)A02243-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSFPQ
- SizePrice
Product group Antibodies
References
SFPQ antibodyGTX112116
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetSFPQ
- SizePrice
Product group Antibodies
SFPQ Polyclonal AntibodyCAC14992
ApplicationsWestern Blot, ELISA
ReactivityMouse
TargetSFPQ
- SizePrice
Product group Antibodies
SFPQ Recombinant Antibody, AbBy Fluor-594 ConjugatedBSM-61746R-BF594
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetSFPQ
- SizePrice
Product group Antibodies
Anti-SFPQ AntibodyA326292
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
- SizePrice
Product group Antibodies
SFPQ antibodyGTX64421
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetSFPQ
- SizePrice
Product group Antibodies
SFPQ antibody [B92]GTX11825
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, RadioImmunoAssay
ReactivityHuman, Mouse
TargetSFPQ
- SizePrice
Product group Antibodies
Goat anti-PSF / SFPQEB09523
ApplicationsELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Mouse, Rat
TargetSFPQ
- SizePrice