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SFPQ Rabbit pAb

A0958
ABclonal
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSFPQ
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Overview

  • Supplier
    ABclonal
  • Product Name
    SFPQ Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB,IHC,IF/ICC,IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6421
  • Target name
    SFPQ
  • Target description
    splicing factor proline and glutamine rich
  • Target synonyms
    POMP100, PPP1R140, PSF, splicing factor, proline- and glutamine-rich, 100 kDa DNA-pairing protein, DNA-binding p52/p100 complex, 100 kDa subunit, PTB-associated splicing factor, epididymis secretory sperm binding protein, polypyrimidine tract binding protein associated, polypyrimidine tract-binding protein-associated splicing factor, protein phosphatase 1, regulatory subunit 140, splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated), splicing factor proline/glutamine-rich
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23246
  • Protein Name
    Splicing factor, proline- and glutamine-rich
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SFPQ using anti-SFPQ antibody (A02243-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse C2C12 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFPQ antigen affinity purified polyclonal antibody (Catalog # A02243-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFPQ at approximately 100 kDa. The expected band size for SFPQ is at 76 kDa.
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