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ProductsBack/SFR1 / C10orf78 Antibody (aa1-30)

SFR1 / C10orf78 Antibody (aa1-30)

LS-C156922
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetSFR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SFR1 / C10orf78 Antibody (aa1-30)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    Flo (1:10 - 1:50), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID119392
  • Target name
    SFR1
  • Target description
    SWI5 dependent homologous recombination repair protein 1
  • Target synonyms
    C10orf78, MEI5, MEIR5, bA373N18.1, swi5-dependent recombination DNA repair protein 1 homolog, MEI5 recombination repair protein homolog, SWI5-dependent recombination repair 1, SWI5-dependent recombination repair protein 1, meiosis protein 5 homolog
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SFR1 using anti-SFR1 antibody (A07683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFR1 antigen affinity purified polyclonal antibody (Catalog # A07683-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFR1 at approximately 22 kDa. The expected band size for SFR1 is at 28 kDa.
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