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SGK1 Polyclonal Antibody

E-AB-60146
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SGK1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SGK1 using anti-SGK1 antibody (A00673-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGK1 antigen affinity purified polyclonal antibody (Catalog # A00673-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGK1 at approximately 55KD. The expected band size for SGK1 is at 55KD.
Product group Antibodies
Boster Bio
Anti-SGK1 Antibody Picoband(r)A00673-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetSGK1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SGK1 Antibody144-01025
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSGK1
  • SizePrice
Product group Antibodies
Biomatik
SGK1 Polyclonal AntibodyCAC13197
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetSGK1
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Formalin-fixed and paraffin embedded human kidney labeled with Anti-Phospho-SGK1 (Ser78) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
Bioss
Phospho-SGK1 (Ser78) Polyclonal AntibodyBS-3395R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat
TargetSGK1
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Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using SGK1 Mouse mAb diluted at 1:200.
Product group Antibodies
Cusabio
SGK1 Monoclonal AntibodyCSB-MA286402
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSGK1
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Product group Antibodies
Assay Genie
TBX2 AntibodyCAB10250
ReactivityHuman
TargetSGK1
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Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
Product group Antibodies
Atlas Antibodies
Anti-SGK1 AntibodyHPA051251
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ReactivityHuman
TargetSGK1
  • SizePrice
Product group Antibodies
Everest Biotech
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ApplicationsELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Mouse, Rat
TargetSGK1
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Product group Antibodies
MedChem Express
SGK1 Antibody (YA1525, Rabbit)HY-P81780
TargetSGK1
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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SGK1 antibody (GTX107750) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
GeneTex
SGK1 antibodyGTX107750
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetSGK1
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