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WB analysis of various sample lysates using GTX66093 SHARPIN antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66093 SHARPIN antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66093 SHARPIN antibody. Dilution : 1:3000 Loading : 25microg per lane

SHARPIN antibody

GTX66093
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetSHARPIN
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Overview

  • Supplier
    GeneTex
  • Product Name
    SHARPIN antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81858
  • Target name
    SHARPIN
  • Target description
    SHANK associated RH domain interactor
  • Target synonyms
    AIFID, SIPL1, sharpin, hSIPL1, shank-associated RH domain-interacting protein, shank-interacting protein-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H0F6
  • Protein Name
    Sharpin
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, LO2 whole cell lysate All lanes: SHARPIN antibody at 5.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 34 kDa Observed band size: 40 kDa
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Figure 1. Western blot analysis of SHARPIN using anti-SHARPIN antibody (A06687-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHARPIN antigen affinity purified polyclonal antibody (A06687-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHARPIN at approximately 40 kDa. The expected band size for SHARPIN is at 40 kDa.
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Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SHARPIN Polyclonal Antibody, Unconjugated (bs-9581R) at 1:400 overnight at 4°C, followed by DAB staining.
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