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ProductsBack/Sheep anti bcl-2

Sheep anti bcl-2

A105P
Nordic-MUbio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
TargetBCL2
250 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Sheep anti bcl-2
  • Delivery Days Customer
    7
  • Application Supplier Note
    Western Blots at 1:5000, Immunoprecipitation - Empirical, Immunofluorescence 1:5000.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Applications Supplier
    Western Blotting;Immunoprecipitation;Immunofluorescense
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID596
  • Target name
    BCL2
  • Target description
    BCL2 apoptosis regulator
  • Target synonyms
    Bcl-2, PPP1R50, apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, protein phosphatase 1, regulatory subunit 50
  • Host
    Sheep
  • Isotype
    IgG
  • Protein IDP10415
  • Protein Name
    Apoptosis regulator Bcl-2
  • Scientific Description
    hbcl2, sheep anti human bcl 2, bcl-2
  • Shelf life instruction
    See expiration date on vial
  • Source
    Sheep were immunized with full length recombinant bcl-2 protein
  • Reactivity Supplier
    Human;Dog;Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCL2 using anti-BCL2 antibody (A00040-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2 antigen affinity purified polyclonal antibody (Catalog # A00040-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2 at approximately 26 kDa. The expected band size for BCL2 is at 26 kDa.
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