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ProductsBack/SHIP (INPP5D) Mouse Monoclonal Antibody [Clone ID: SHIP-01]
Western Blotting analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control Lane 2, 3: immunostaining with anti-human SHIP-1 (SHIP-01) Lane 4, 5: immunostaining with anti-human SHIP-1 (SHIP-02)
Western Blotting analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control Lane 2, 3: immunostaining with anti-human SHIP-1 (SHIP-01) Lane 4, 5: immunostaining with anti-human SHIP-1 (SHIP-02)
Western Blotting analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control Lane 2, 3: immunostaining with anti-human SHIP-1 (SHIP-01) Lane 4, 5: immunostaining with anti-human SHIP-1 (SHIP-02)

SHIP (INPP5D) Mouse Monoclonal Antibody [Clone ID: SHIP-01]

AM03030PU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetINPP5D
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SHIP (INPP5D) Mouse Monoclonal Antibody [Clone ID: SHIP-01]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SHIP-01
  • Gene ID3635
  • Target name
    INPP5D
  • Target description
    inositol polyphosphate-5-phosphatase D
  • Target synonyms
    SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship, phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1, SH2 domain-containing inositol 5'-phosphatase 1, inositol polyphosphate-5-phosphatase, 145kD, inositol polyphosphate-5-phosphatase, 145kDa, phosphatidylinositol 4,5-bisphosphate 5-phosphatase, signaling inositol polyphosphate 5 phosphatase SIP-145, signaling inositol polyphosphate phosphatase SHIP II
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ92835
  • Protein Name
    Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
  • Scientific Description
    SHIP (INPP5D) mouse monoclonal antibody, clone SHIP-01, Aff - Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of INPP5D using anti-INPP5D antibody (A03358-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INPP5D antigen affinity purified polyclonal antibody (Catalog # A03358-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for INPP5D at approximately 145 kDa. The expected band size for INPP5D is at 133 kDa.
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