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SHOC2 antibody

GTX130206
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSHOC2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SHOC2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.38 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8036
  • Target name
    SHOC2
  • Target description
    SHOC2 leucine rich repeat scaffold protein
  • Target synonyms
    leucine-rich repeat protein SHOC-2; NSLH1; SIAA0862; SOC2; soc-2 suppressor of clear homolog; SUR8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQ13
  • Protein Name
    Leucine-rich repeat protein SHOC-2
  • Scientific Description
    This gene encodes a protein that consists almost entirely of leucine-rich repeats, a domain implicated in protein-protein interactions. The protein may function as a scaffold linking RAS to downstream signal transducers in the RAS/ERK MAP kinase signaling cascade. Mutations in this gene have been associated with Noonan-like syndrome with loose anagen hair. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Shoc2/Sur8 using anti-Shoc2/Sur8 antibody (A07214-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Shoc2/Sur8 antigen affinity purified polyclonal antibody (Catalog # A07214-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Shoc2/Sur8 at approximately 70 kDa. The expected band size for Shoc2/Sur8 is at 70 kDa.
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